分子克隆实验指南（第四版）

　　Molecular Cloning has served as thefoundation of technical expertise in labs worldwide for 30 years.No other manual has been so popular， or so influential. MolecularCloning， Fourth Edition， by the celebrated founding author JoeSambrook and new co-author， the distinguished HHMI investigatorMichael Green， preserves the highly praised detail and clarity ofprevious editions and includes specific chapters and protocolscommissioned for the book from expert practitioners at Yale， UMass， Rockefeller University， Texas Tech， Cold Spring HarborLaboratory， Washington University， and other leading institutions.The theoretical and historical underpinnings of techniques areprominent features of the presentation throughout， information thatdoes much to help trouble-shoot experimental problems.For the fourth edition of this classic work，the content has been entirely recast to include nucleic-acid basedmethods selected as the most widely used and valuable in molecularand cellular biology laboratories.Core chapters from the third edition havebeen revised to feature current strategies and approaches to thepreparation and cloning of nucleic acids， gene transfer， andexpression analysis. They are augmented by 12 new chapters whichshow how DNA， RNA， and proteins should be prepared， evaluated， andmanipulated， and how data generation and analysis can behandled.The new content includes methods for studyinginteractions between cellular components， such as microarrays，next-generation sequencing technologies， RNA interference， andepigenetic analysis using DNA methylation techniques and chromatinimmunoprecipitation. To make sense of the wealth of data producedby these techniques， a bioinformatics chapter describes the use ofanalytical tools for comparing sequences of genes and proteins andidentifying common expression patterns among sets of genes.Building on thirty years of trust， reliability，and authority， the fourth edition of Molecular Cloning is the newgold standard—the one indispensable molecular biology laboratorymanual and reference source. 新版特点：？ Extensive new content： 12 entirely new chapters are devoted tothe most exciting current research strategies， including epigeneticanalysis， RNA interference， genome sequencing， andbioinformatics？ Expanded scope： the nucleic-acid-based techniques selected forinclusion have promoted recent advances in gene transfer， proteinexpression， RNA analysis， and expression of cloned genes？ Classic content： 10 original core chapters have been updatedto reflect developments and innovations in standard techniques andto introduce new cutting-edge protocols？ Easy-to-follow format： the previous editions’ renowned attentionto detail and accuracy are fully retained？ Essential appendices： an up-to-date collection of reagents，vectors， media， detection systems， and commonly used techniques areincluded？ Expanded authorship： chapters and protocols have beenspecifically commissioned from renowned experts at leadinginstitutions

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